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primary antibody against proliferating cell nuclear antigen (pcna; wl02208)  (Wanleibio)

 
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    Wanleibio primary antibody against proliferating cell nuclear antigen (pcna; wl02208)
    Primary Antibody Against Proliferating Cell Nuclear Antigen (Pcna; Wl02208), supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+against+proliferating+cell+nuclear+antigen+(pcna%3B+wl02208)/primary+antibodies+against+pcna/pm40103007-85-16-22
    Average 90 stars, based on 1 article reviews
    primary antibody against proliferating cell nuclear antigen (pcna; wl02208) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-170165, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. Cell counting Kit-8 (CCK-8) array A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-1701-65, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Membrane:

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-170165, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. Cell counting Kit-8 (CCK-8) array A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-1701-65, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Incubation:

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-170165, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. Cell counting Kit-8 (CCK-8) array A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease
    Article Snippet: Protein samples were separated via SDS‒PAGE and transferred to polyvinylidene fluoride membranes (Millipore). .. After blocking with 5% fat-free milk for 2 h, the membrane strips were incubated with primary antibodies against PCNA (1:1000; WL02208, Wanleibio, China), p-mTOR (1:2000; ab109268, Abcam), mTOR (1:2000; #2972 CST), p62 (1:2000; ab109012, Abcam), LC3B (1:1000; et-1701-65, HUABIO) and β-actin (1:2000; 60008-1-lg, Proteintech) at 4 °C overnight, followed by incubation with an HRP-labeled secondary antibody at 25 °C for 1 h. The immunoreactivity of each protein band was detected via an enhanced chemiluminescence kit (ECL) (MA0186, Meilunbio, Dalian8-1-lg) under an automatic chemiluminescence image analysis system (Tanon, Shanghai, China). .. A CCK-8 assay was conducted to detect cell viability according to the manufacturer’s instructions (C0038, Beyotime).

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: .. After endogenous peroxidase activity was blocked, the renal sections were incubated with primary antibody against proliferating cell nuclear antigen (PCNA; 1:100; WL02208, Wanleibio, China) overnight at 4 °C and then analyzed via a universal two-step detection kit (mouse/rabbit enhanced polymer detection system) (PV-9000, ZSGB-BIO, Beijing, China) according to the manufacturer’s protocol. ..

    Activity Assay:

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: .. After endogenous peroxidase activity was blocked, the renal sections were incubated with primary antibody against proliferating cell nuclear antigen (PCNA; 1:100; WL02208, Wanleibio, China) overnight at 4 °C and then analyzed via a universal two-step detection kit (mouse/rabbit enhanced polymer detection system) (PV-9000, ZSGB-BIO, Beijing, China) according to the manufacturer’s protocol. ..

    Polymer:

    Article Title: Extracellular vesicles derived from mesenchymal stem cells alleviate renal fibrosis via the miR-99b-5p/mTOR/autophagy axis in diabetic kidney disease.
    Article Snippet: .. After endogenous peroxidase activity was blocked, the renal sections were incubated with primary antibody against proliferating cell nuclear antigen (PCNA; 1:100; WL02208, Wanleibio, China) overnight at 4 °C and then analyzed via a universal two-step detection kit (mouse/rabbit enhanced polymer detection system) (PV-9000, ZSGB-BIO, Beijing, China) according to the manufacturer’s protocol. ..



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    primary antibody against proliferating cell nuclear antigen (pcna; wl02208)  (Wanleibio)
    90
    Wanleibio primary antibody against proliferating cell nuclear antigen (pcna; wl02208)
    Primary Antibody Against Proliferating Cell Nuclear Antigen (Pcna; Wl02208), supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+against+proliferating+cell+nuclear+antigen+(pcna%3B+wl02208)/primary+antibodies+against+pcna/pm40103007-85-16-22
    Average 90 stars, based on 1 article reviews
    primary antibody against proliferating cell nuclear antigen (pcna; wl02208) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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